Distribution of 35S in Subcellular Components After Administration of Thiamine Propyl Disulfide-35S (inner)

The preparation of [35S]homocysteine thiolactone free of [35S]methionine.

The unambiguous study of homocysteine metabolism requires a source of [355]homocysteine completely free from contaminating [35S]methionine. Until now, such a reagent has not been readily available. Homocysteine is an important metabolite involved in methionine, cysteine and methylation metabolism. Homocysteine can be methylated to form methionine with either 5-methyltetrahydrofolate [1] or beta...

Expression of ricin under control of three different promoters, Cauliflower mosaic virus 35S promoter (35S), dual enhanced Cauliflower mosaic virus 35S promoter (35S)

Affinity of Thiamine Propyl Disulfide-s35 to Blood.

The fact that the tissue affinity of thiamine propyl disulfide (TPD) is superior to that of thiamin hydrochloride has been shown by many studies in this country as well as in foreign countries. Especially, the distribution of thiamine in the blood after administration of TPD is worthy of note, i.e., the thiamine concentra tion in the blood, particularly in blood cells, is markedly higher after ...

Effects of chronic morphine administration on mu opioid receptor-stimulated [35S]GTPgammaS autoradiography in rat brain.

Chronic opiate administration results in the development of tolerance and dependence, but the regulation of mu opioid receptor function during this process is not clearly understood. To localize changes in mu opioid receptor-coupled G-protein activity in various brain regions after chronic morphine treatment, the present study examined mu opioid agonist-stimulated [35S]GTPgammaS binding to brai...

Effect of avitaminosis E on the incorporation of 35S-sulfate by rat tissue components.